Product Description
We are delivering an optimum range of 6X Gel Loading Buffer. It is used as a loading dye for visual tracking of DNA migration during electrophoresis. This buffer is formulated to facilitate loading of DNA containing samples into the wells of agarose. Moreover, provided buffer is used to prepare DNA markers. 6X Gel Loading Buffer is a great sample solution to give a final concentration. It is to load DNA sample without loading any buffer.
Premium Quality & Convenient PackagingOffered in 1 mL, 5 mL, 10 mL, and bulk packs, the buffer comes in secure, graduated vials to prevent leaks and ensure precise dispensing. Each vial accommodates various usage-settings in laboratories, making it a versatile choice for distributors, exporters, manufacturers, and suppliers.
Efficient Sample Preparation for Reliable ResultsBlend 1 part of 6X Gel Loading Buffer with five parts nucleic acid sample to obtain the ideal density and migration visibility. Its Tris-HCl buffering capability maintains sample integrity, and Bromophenol Blue offers straightforward loading and tracking during electrophoresis runs.
FAQ's of 6X Gel Loading Buffer:
Q: How do I properly mix 6X Gel Loading Buffer with my sample before agarose gel electrophoresis?
A: To prepare samples, mix 1 part of the 6X Gel Loading Buffer with 5 parts of your DNA or RNA solution. This dilution yields a 1X working concentration suitable for direct loading onto agarose or polyacrylamide gels.
Q: What are the main benefits of using this buffer for nucleic acid electrophoresis?
A: The buffer ensures consistent sample density for even migration, provides vivid color for loading precision, and contains EDTA, which protects nucleic acids by chelating metal ions. Its Tris-HCl composition stabilizes pH during electrophoresis.
Q: When should the buffer be diluted, and can it be added directly to samples?
A: Always dilute the 6X buffer immediately before sample loading. Use one part buffer to five parts sample to ensure effective performance and visibility during electrophoresis.
Q: Where should I store the 6X Gel Loading Buffer to maintain its stability and shelf life?
A: Store the buffer at 2C-8C, ideally in the dark, to maximize its shelf life of up to 24 months and preserve its molecular integrity.
Q: What makes this buffer compatible with both DNA and RNA applications?
A: The buffer's neutral pH (~7.6), the presence of Tris-HCl, and chelating EDTA make it suitable for both DNA and RNA electrophoresis, maintaining sample stability and performance across different nucleic acid types.
Q: Is the buffer safe, and does it have any odor or toxic properties?
A: 6X Gel Loading Buffer is non-poisonous, odorless, and meets molecular biology grade standards, ensuring it is safe for typical laboratory use without special handling concerns.