Product Description
We are successfully engaged in providing a broad variety of Laemmli SDS Sample Buffer 5X. It is used as a protein sample loading buffer for use in SDS-PAGE. Apart from this, provided range is able to facilitate the loading of samples into the wells of the gel. Laemmli SDS Sample Buffer 5X is most commonly used sample buffer for Sodium Dodecyl Sulfate. It is also considering as crucial buffer in the preparation of protein samples.
Specification
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Brand
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Aura Biotechnologies
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Country of Origin
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Made in India
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Usage/Application
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Laboratory
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Grade Standard
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Bio-Tech Grade
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PH Level
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8.0 pH
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Packaging Type
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Bottle
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Packaging Size
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100 ml
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Optimal Protein DenaturationThe Laemmli SDS Sample Buffer 5X ensures effective denaturation of protein samples for reliable and reproducible SDS-PAGE results. Its balanced composition, including reducing agents and tracking dye, creates ideal conditions for separation and visualization of proteins.
Convenient, Ready-to-Use SolutionThis buffer is supplied pre-mixed and ready for immediate use, eliminating the need for on-site preparation and minimizing the risk of errors. Simply add to your samples, mix briefly, and proceed with heating and loading, streamlining your workflow.
Superior Stability and Packaging FlexibilityPackaged in 10 mL, 50 mL, 100 mL, and customizable volumes, our buffer remains stable for up to 12 months when stored at -20C. Each container is pre-labeled with the product code and lot number, facilitating traceability and inventory management.
FAQ's of Laemmli SDS Sample Buffer 5X:
Q: How do I use Laemmli SDS Sample Buffer 5X for protein sample preparation?
A: Simply mix the buffer with your protein sample, heat the mixture at 95C for a few minutes to ensure complete denaturation, then load onto the gel for SDS-PAGE analysis.
Q: What is the benefit of using this buffer in SDS-PAGE electrophoresis?
A: It provides effective denaturation and ensures uniform sample loading, enhancing protein separation accuracy and resulting in clear, distinct bands during electrophoresis.
Q: When should I prepare my samples using the buffer before running SDS-PAGE?
A: Samples should be prepared with the buffer immediately prior to SDS-PAGE. Mixing and heating just before loading delivers optimal results and maintains sample integrity.
Q: Where should I store the Laemmli SDS Sample Buffer 5X, and for how long?
A: Store the buffer at -20C. It retains stability and efficacy for up to 12 months under recommended storage conditions.
Q: Is the buffer compatible with all types of protein electrophoresis?
A: Yes, it is compatible with most standard SDS-PAGE protocols and widely used in molecular biology research for protein analysis.
Q: What safety and labeling assurances come with this buffer?
A: It is non-toxic, odorless, pre-labeled with batch and lot information for accurate identification, and intended solely for research purposes.