Product Description
We are providing a wide range ofTris Borate Edta 10X to our clients. This range is used in nucleic acid electrophoresis. Therefore, provided range is able to conduct electricity because of their concentration of hydrogen ion. It is also routinely used for DNA automated sequencing gel. Tris Borate Edta 10X is a solution used in Agarose Gel Electrophoresis to separate the nucleic acids. It is especially suitable for the agarose gel electrophoresis of human papillomavirus DNA.
Reliable Buffer for Nucleic Acid ElectrophoresisSelect TBE 10X Buffer for precise and reproducible results in electrophoresis applications involving DNA and RNA. Its high molecular biology grade and excellent purity make it a preferred solution for preparing agarose and polyacrylamide gels, ensuring efficient sample migration and resolution in electrophoresis.
Versatile, Convenient Packaging OptionsTBE Buffer is available in several packaging sizes500 ml, 1 L, and 5 Lmeeting varied laboratory requirements. Bottled in durable HDPE containers, it maintains buffer integrity during storage and handling. Custom packaging is also available to suit specific research or industrial needs.
Stable, Safe, and Easy to PrepareCompletely soluble and odorless, TBE Buffer is non-hazardous, making it safe for routine lab handling. Its stability and pH consistency at 25C guarantee reliable performance throughout its 36-month shelf life, provided it is stored away from light at room temperature.
FAQs of Tris Borate Edta 10X:
Q: How should I prepare working strength TBE buffer from the 10X concentrate?
A: To prepare a 1X working solution, simply dilute one part of the 10X TBE buffer with nine parts deionized water. Mix thoroughly until fully homogeneousfor example, mix 100 ml of 10X buffer with 900 ml of water.
Q: What are the main applications of Tris Borate EDTA 10X buffer?
A: TBE 10X buffer is commonly used in molecular biology, specifically for the electrophoresis of nucleic acids such as DNA or RNA in agarose and polyacrylamide gels. It ensures optimal migration and resolution of nucleic acid samples during electrophoresis.
Q: When should I use TBE buffer instead of other electrophoresis buffers?
A: TBE buffer is preferred when working with high voltage electrophoresis or when excellent resolution of small DNA fragments is required. Its consistent buffering capacity and compatibility with DNA/RNA make it ideal for molecular biology and analytical applications.
Q: Where should I store TBE buffer for optimal stability?
A: Store TBE buffer at room temperature and keep the container tightly closed, away from direct sunlight. Proper storage will help maintain the buffers stability and performance for up to 36 months.
Q: What are the benefits of using TBE buffer for nucleic acid electrophoresis?
A: Using TBE buffer ensures stable pH, excellent conductivity, and high sample resolution. It is odorless, non-hazardous, and fully compatible with DNA/RNA, providing safe, reliable, and reproducible results in electrophoresis experiments.
Q: Is TBE buffer safe to handle in the laboratory environment?
A: Yes, TBE buffer is classified as non-hazardous and is odorless, making it suitable and safe for normal laboratory conditions when handled according to standard protocols.
Q: Can I use TBE buffer with both agarose and polyacrylamide gels?
A: Absolutely. TBE buffer is specifically formulated for use in both agarose and polyacrylamide gels, making it a versatile choice for various electrophoresis protocols involving nucleic acids.