Product Description
Our organization is offering an assorted variety of 500 ML Phosphate Buffered Saline 10X. It is to maintain a constant Ph. This range is used as a wash and dilution buffer. It to take a reference spectrum when measuring protein adsorption. 500 ML Phosphate Buffered Saline 10X is found in laboratory protocols for dilutions as well as a balanced solution that is normally used for a wider range of cell culture applications.
Ideal Buffer for Research LaboratoriesThis PBS 10X solution is engineered to meet the rigorous demands of cell culture, biological, and biochemical research labs. Its clarity, high purity (typically 99%), and reliable pH maintain scientific consistency in experimental protocols. Supplied in a robust 500 mL HDPE bottle, it's easy to transport and store at room temperature, minimizing logistical challenges.
Simple Preparation for Routine UseThe concentrated PBS is completely soluble and odorless, designed for straightforward dilution to 1X strength using sterile distilled water. This ensures ease of use across various applications, including cell washing and tissue rinsing. The clear liquid form allows for direct visual inspection and quality assurance before employing in sensitive procedures.
FAQ's of 500 ML Phosphate Buffered Saline 10X:
Q: How should I prepare 1X PBS from the 10X concentrate?
A: To create 1X PBS, dilute the 10X concentrate with sterile distilled water at a ratio of 1:9. For example, mix 50 mL of PBS 10X with 450 mL of water to obtain 500 mL of 1X PBS, ensuring thorough mixing for complete solubility.
Q: What are the common applications of PBS 10X in research?
A: PBS 10X is widely used in biological and biochemical research, especially for cell washing, tissue rinsing, and as a buffer solution in a variety of laboratory protocols involving cells and tissues.
Q: When should this PBS 10X solution be used within its shelf life?
A: For optimal quality and reliability, use the PBS 10X solution within 24 months from its date of manufacture, as indicated on the bottle label or accompanying documentation.
Q: Where can I store the PBS 10X solution, and what conditions should I avoid?
A: Store the PBS 10X solution at room temperature, ideally between 15-30C. Avoid exposing the bottle to freezing temperatures or direct sunlight to maintain chemical integrity and performance.
Q: What is the benefit of using a 10X concentrated PBS over a ready-to-use solution?
A: A 10X concentrate saves storage space and simplifies inventory management. It allows users to prepare fresh, correctly diluted PBS as needed, thereby reducing the risk of contamination and ensuring buffer stability.
Q: Is the PBS 10X sterile or suitable for cell culture?
A: This specific PBS 10X is non-sterile. For cell culture applications requiring sterility, dilute using sterile distilled water and, if needed, sterilize the solution before use.
Q: How can I obtain the lot number for a specific bottle of PBS 10X?
A: The batch number, expiry date, and storage conditions are displayed on each bottle. Lot numbers are available on request from the supplier or manufacturer.