Product Description
Owing to rapid growth in this business realm, we are offering a unique array of Ultra Elisa Coating Buffer. It provides the optimal pH and ionic strength for antigen binding. This buffer is helpful to preserve three dimensional structure. Ultra Elisa Coating Buffer is able to give a greater binding reactivity with the detection molecule. It is crucial for efficient immobilization. It is used to coat the ELISA multiwell plate and optimizing interactions with the detection antibody.
Specification
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Grade Standard
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Laboratory
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Usage/Application
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Scientific Research
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Brand
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Aura Biotechnologies
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Physical State
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Liquid
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Packaging Type
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Bottle
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Packaging Size
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100 ml
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Optimized for Peak ELISA PerformanceThis buffers optimized pH level and proprietary formulation maximize antigen or antibody adsorption on microplate wells. It delivers reliable results in sensitive ELISA and immunoassay applications, supporting high sensitivity and reproducibility, crucial for consistent laboratory data.
Versatile and Compatible ApplicationUltra ELISA Coating Buffer is compatible with a wide range of standard polystyrene microplates. Its clear, odorless nature ensures no interference in visual or spectrometric readouts. The buffer can be used straight from the bottle or sterilized by filtration, offering flexibility according to laboratory needs.
Stringent Quality and StabilityUnder strict lot-to-lot quality control, Ultra ELISA Coating Buffer provides analytical-grade purity (99%) and low endotoxin content. With a shelf life of 12 months at 28C, it maintains its performance and integrity throughout storage and multiple assay cycles.
FAQs of Ultra Elisa Coating Buffer:
Q: How should I store Ultra ELISA Coating Buffer to maintain its effectiveness?
A: Store the buffer at 28C in a tightly sealed container. Avoid repeated freeze-thaw cycles and prepare it for use by allowing it to reach room temperature before application.
Q: What is the recommended process for using this buffer in ELISA plate coating?
A: Equilibrate the buffer to room temperature, add it to microplate wells along with your antigen or antibody, and incubate as per your protocol for optimal adsorption. If sterility is required, filter-sterilize before use.
Q: When is filter sterilization advised for this buffer?
A: Filter sterilization is recommended when working with sensitive assays that require aseptic conditions or when using the buffer in applications where contamination must be strictly controlled.
Q: Where can this buffer be applied beyond standard ELISA?
A: Besides ELISA, it is suitable for other immunoassays that involve coating proteins, peptides, or antibodies on polystyrene surfaces, expanding its utility across various laboratory techniques.
Q: What are the primary benefits of Ultra ELISA Coating Buffer compared to typical coating buffers?
A: Its low endotoxin level, optimized pH, high purity, and lot-to-lot consistency reduce background interference and assay variability, leading to more reliable and reproducible results.
Q: How does the buffers compatibility enhance laboratory workflow?
A: It is ready-to-use and fully miscible in water, streamlining preparation steps and ensuring consistent performance with standard ELISA microplates, saving preparation time and reducing experimental error.