Heat Labile Uracil DNA Glycosylase

Name: Heat Labile Uracil DNA Glycosylase
Brand: AURA BIOTECHNOLOGIES PRIVATE LIMITED
Price: 5000 INR
Availability: InStock

5000 INR/Piece

Get a Price/Quote

Product Details:

Shelf Life 12 Months
Accuracy High substrate specificity
Storage Instructions Store at -20C
Instruments Type DNA Repair & PCR Reagents
Features Heat labile, high specificity, active at low temperature, rapid
Usage Type Laboratory/Research
Frequency Single Use/Batch
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Heat Labile Uracil DNA Glycosylase Price And Quantity

Price 5000 INR/Piece

Package Size 50U, 250U, 500U, 1000U per vial
Optimal Temperature 37C (Inactivated at 50C within minutes)
Form Liquid or lyophilized
Enzyme Source E. coli expression system
Activity 1 Unit/L
CAS Number 9027-34-7
Purity >99% (SDS-PAGE)
Application Prevention of carryover contamination in PCR
Buffer Compatibility Compatible with standard PCR buffers
Unit Definition Amount required to release 1 nmol of uracil from DNA in 1 hour at 37C
Safety Information Non-hazardous
Inactivation Method Heat (50C for 10 minutes)

Heat Labile Uracil DNA Glycosylase Product Specifications

Condition New
Shelf Life 12 Months
Use Removes uracil residues from DNA
Usage Type Laboratory/Research
Material Recombinant Protein
Real-Time Operation Yes
Operating Type Manual and Automated
Storage Instructions Store at -20C
Accuracy High substrate specificity
Equipment Type Enzyme/Kit
Frequency Single Use/Batch
Noise Level Silent
Portable Yes
Instruments Type DNA Repair & PCR Reagents
Color Colorless/Transparent
Function Removes uracil from double- and single-stranded DNA
Dimension (L*W*H) Custom/Varies by Pack Size
Wall Mounted No
Features Heat labile, high specificity, active at low temperature, rapid
Weight Few Milligrams (enzyme) per vial
Technology Enzymatic DNA Modification

Package Size 50U, 250U, 500U, 1000U per vial
Optimal Temperature 37C (Inactivated at 50C within minutes)
Form Liquid or lyophilized
Enzyme Source E. coli expression system
Activity 1 Unit/L
CAS Number 9027-34-7
Purity >99% (SDS-PAGE)
Application Prevention of carryover contamination in PCR
Buffer Compatibility Compatible with standard PCR buffers
Unit Definition Amount required to release 1 nmol of uracil from DNA in 1 hour at 37C
Safety Information Non-hazardous
Inactivation Method Heat (50C for 10 minutes)

Heat Labile Uracil DNA Glycosylase Trade Information

Payment Terms Cash in Advance (CID), Cheque, Cash Advance (CA)
Main Domestic Market All India

Product Description

By rupturing the N-glycosidic link connecting the uracil base and the sugar backbone, glycosylase removes uracil from DNA that contains duplex uracil. By excising uracil and generating alkali-sensitive abasic sites in the DNA, this Heat Labile Uracil DNA Glycosylase hydrolyzes uracil-glycosidic linkages in single- or double-stranded DNA. The addition of EDTA has no effect on the activity of Heat Labile Uracil DNA Glycosylase since it does not require metal ions. It is able to cleave U-DNA in a general, site- or strand-specific, or a combination of these ways.

Effective PCR Contamination Control

UDG removes incorporated uracil from DNA, preventing unwanted amplification of carryover contaminants. Its high substrate specificity and heat-labile property ensure efficient activity during setup and easy inactivation before amplification, safeguarding PCR integrity.

Flexible Formats and High Purity

Available as a liquid or lyophilized enzyme, UDG suits various laboratory workflows. With over 99% purity, it guarantees minimal impurities for reliable performance, and its compatibility with standard PCR buffers allows seamless integration into existing protocols.

FAQ's of Heat Labile Uracil DNA Glycosylase:

Q: How is Heat Labile Uracil DNA Glycosylase used to prevent PCR carryover contamination?

A: The enzyme specifically removes uracil residues from DNA, rendering contaminating uracil-containing DNA templates non-amplifiable. Add UDG to reaction mixes before amplification, incubate at 37C, and then heat-inactivate at 50C for 10 minutes before starting PCR cycling.

Q: What makes this enzyme heat labile, and why is that beneficial?

A: This recombinant UDG is inactivated within minutes at 50C, allowing selective removal of uracil before PCR while ensuring the enzyme does not degrade newly synthesized DNA during thermal cycling, thus enhancing specificity and workflow efficiency.

Q: When should I add UDG to my reaction, and how do I inactivate it?

A: Introduce UDG during PCR reaction setup, incubate at 37C for the required time, then heat the mixture at 50C for 10 minutes to inactivate the enzyme before initiating the amplification phase.

Q: Where can this enzyme be stored, and what is its shelf life?

A: Store UDG at -20C as recommended to maintain stability and activity. The enzyme has a shelf life of up to 12 months when kept at the proper storage temperature.

Q: What pack sizes are offered, and how much enzyme is in each vial?

A: The enzyme is available in 50U, 250U, 500U, and 1000U per vial. Actual enzyme weight per vial is a few milligrams, varying by quantity purchased.

Q: How does UDG function at the molecular level?

A: UDG cleaves the N-glycosidic bond between uracil and sugar-phosphate DNA backbone, releasing free uracil from double- or single-stranded DNA and facilitating the removal of unwanted uracil bases.

Q: What are the main benefits of using this heat labile UDG in the laboratory?

A: Using this enzyme ensures high accuracy in PCR by effectively removing uracil residues to prevent false positives from carryover DNA. Its heat-labile property streamlines workflows, and its compatibility with standard buffers ensures broad applicability across laboratory setups.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Corona Test Kit' category

Hot Start Taq DNA Polymerase PCR Price : 7000 INR/Unit Real-Time Operation : Other, Compatible Material : Other, Recombinant Taq DNA polymerase, stabilizers, buffer components Usage Type : Research laboratory Use : DNA amplification, PCR Shelf Life : 1 year (when stored properly) Condition : New Portable : Yes Equipment Type : Hot Start Taq DNA Polymerase PCR Accuracy : High fidelity for PCR applications Operating Type : Other, Manual or automated thermocycler Storage Instructions : Store at 20C for longterm stability Function : Selective DNA amplification Color : White (reagent), Colorless (enzyme) Instruments Type : Molecular biology reagent Technology : Hot Start PCR Weight : Varies by volume (11000 Units format) Features : High specificity, reduced nonspecific amplification, optimal yield Wall Mounted : No Dimension (L*W*H) : Supplied in microcentrifuge tubes (varied kit sizes) Measurement Range : Fragment sizes from 100 bp to 4 kb Noise Level : Silent (biochemical usage) Power Source : Other, Not required (reagentbased)

Send Inquiry

Magnetic Beads for Rna-Dna Extraction Kit Price : 1000 INR/Pack Real-Time Operation : Yes Material : Other, Superparamagnetic nanoparticles with silica coating Usage Type : Laboratory Use Only Use : Nucleic Acid (RNA/DNA) Extraction and Purification Shelf Life : 1224 Months Condition : New Portable : Yes Equipment Type : Magnetic Beads for RNADNA Extraction Kit Accuracy : High yield and purity (>95%) Operating Type : Other, Manual/Automated Compatible Storage Instructions : Store at 28C, protect from sunlight and freezing Function : Efficient isolation of total RNA and genomic DNA from biological samples Color : Brown/Black Instruments Type : Extraction Kit Consumable Technology : Magnetic Separation Weight : Varies per pack (typically 10 g 500 g) Features : High binding capacity, rapid separation; suitable for highthroughput extraction; compatible with automated systems Wall Mounted : No Dimension (L*W*H) : Custom Packaging (Usually 550 mL packaging available) Measurement Range : 200 bp to 50 kb fragments Noise Level : Silent Operation Power Source : Other, Not Required (Beads, not instrument)

Send Inquiry

M Mulv Reverse Transcriptase Price : 10000 INR/Piece Real-Time Operation : Other, Suitable for cDNA synthesis protocols Material : Other, Not Applicable (Biological molecule) Usage Type : Laboratory / Research use only Use : Synthesis of complementary DNA (cDNA) from RNA template Shelf Life : Up to 1 year at 20C Condition : New Portable : Yes (can be transferred in laboratory conditions) Equipment Type : Enzyme / Biochemical reagent Accuracy : High fidelity in cDNA synthesis Operating Type : Other, Manual (used in molecular biology workflow) Storage Instructions : Store at 20C, avoid repeated freezethaw cycles Function : Reverse transcribes RNA to DNA Color : Colorless (buffer solution) Instruments Type : Enzyme Technology : Reverse Transcription Weight : Typically 200 Units supplied (biochemical quantity, not physical weight) Features : High efficiency, robust performance, suitable for RTPCR and cDNA synthesis Wall Mounted : No Dimension (L*W*H) : Not Applicable (Supplied in microcentrifuge tubes) Measurement Range : Up to 10 kb cDNA synthesis

Send Inquiry

DNTP Mix Price : 5000 INR/Piece Real-Time Operation : Yes Material : Other, Biochemical Enzyme Solution Usage Type : Laboratory Use Use : Molecular Biology (PCR/RTPCR) Shelf Life : 2 Years Condition : New Portable : Yes Equipment Type : Enzyme Mix / Reagent Accuracy : High, Analytical Grade Operating Type : Manual Storage Instructions : Store at 20C Function : Provides nucleotides for DNA synthesis Color : Colorless/Transparent Instruments Type : Reagent for PCR Instruments Technology : PCR (Polymerase Chain Reaction) Weight : Varies per Packaging (Typically ~1ml/vial) Features : Readytouse dNTP mix, High purity, Optimized for PCR Wall Mounted : No Dimension (L*W*H) : Standard Vial/Bottle Measurement Range : dNTPs Concentration per protocol

Send Inquiry

SEND INQUIRY CALL ME FREE