By rupturing the N-glycosidic link connecting the uracil base and the sugar backbone, glycosylase removes uracil from DNA that contains duplex uracil. By excising uracil and generating alkali-sensitive abasic sites in the DNA, this Heat Labile Uracil DNA Glycosylase hydrolyzes uracil-glycosidic linkages in single- or double-stranded DNA. The addition of EDTA has no effect on the activity of Heat Labile Uracil DNA Glycosylase since it does not require metal ions. It is able to cleave U-DNA in a general, site- or strand-specific, or a combination of these ways.