Product Description
Our firm is highly esteemed in providing a wide variety of M Mulv Reverse Transcriptase. It can synthesize a complementary DNA strand initiating from a primer. This range is used to produce complementary DNA from various expressed mRNAs. It serves as a starting point for synthesis of a new strand. M Mulv Reverse Transcriptase is suitable to achieve reverse transcription of RNA. It comes in varied specifications and modifications as per the needs of consumers.
Reliable cDNA Synthesis and RT-PCRThe M Mulv Reverse Transcriptase ensures efficient and accurate synthesis of cDNA, a critical step for downstream applications like RT-PCR and gene expression profiling. Its ability to reverse transcribe a wide range of RNA templates, combined with high fidelity and low RNase H activity, makes it ideal for molecular biology laboratories seeking consistent performance in their experiments.
High Purity and Batch ConsistencyEach batch of M Mulv Reverse Transcriptase is verified for purity (>95% by SDS-PAGE) and undergoes rigorous lot-to-lot consistency testing. Researchers can rely on its quality across experiments, eliminating concerns of variability and ensuring reproducible results for cloning and diagnostic protocols.
User-Centric Features and Easy HandlingSupplied as a colorless, ready-to-use liquid in a compatible buffer and packaged with 200 units per vial, the enzyme streamlines laboratory workflow. Its portability, stability (up to 1 year at -20C), and compatibility with RNase inhibitor further minimize experimental error and simplify handling during manual operations.
FAQ's of M Mulv Reverse Transcriptase:
Q: How should I store M Mulv Reverse Transcriptase to maintain enzyme activity?
A: To preserve enzyme activity and ensure a long shelf life, store the enzyme at -20C in its supplied buffer. Avoid repeated freeze-thaw cycles to prevent degradation.
Q: What types of RNA templates are compatible with this reverse transcriptase?
A: M Mulv Reverse Transcriptase is compatible with total RNA, poly(A)+ RNA, and viral RNA, making it versatile for various molecular biology applications including cDNA synthesis and RT-PCR.
Q: When is RNase inhibitor recommended for use with this enzyme?
A: RNase inhibitor is recommended during reaction setup to protect RNA templates from degradation, thereby ensuring optimal cDNA synthesis and accurate experimental results.
Q: Where can I use M Mulv Reverse Transcriptase in my laboratory workflow?
A: This enzyme is suitable for use in standard molecular biology procedures, such as first-strand cDNA synthesis, RT-PCR amplification, gene cloning, and gene expression analysis workflows.
Q: What is the process for generating cDNA using this enzyme?
A: The process involves mixing RNA template with M Mulv Reverse Transcriptase in the supplied buffer, optionally with RNase inhibitor, and incubating the reaction at the optimal temperature of 37C for efficient cDNA synthesis.
Q: What benefits does M Mulv Reverse Transcriptase offer compared to other enzymes?
A: This enzyme provides high efficiency, reduced RNase H activity, robust performance across a broad measurement range (up to 10 kb cDNA), and high purity, ensuring reliable results for demanding applications.
Q: Is this product suitable for research use and how is it supplied?
A: M Mulv Reverse Transcriptase is intended for laboratory and research use only, supplied as a liquid enzyme in microcentrifuge tubes containing 200 units per vial and shipped on dry ice to maintain its integrity.