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Pfu Dna Polymerase Vial
3150 INR/Bottle
Product Details:
- Shelf Life 12-24 months (when stored properly)
- Usage Type Laboratory reagent
- Features High accuracy, proof-reading activity, thermostable enzyme
- Accuracy High (35 exonuclease activity ensures low error rate)
- Measurement Range Up to 10 kb DNA fragments amplification
- Function DNA polymerization during PCR
- Storage Instructions Store at -20C
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Pfu Dna Polymerase Vial Price And Quantity
- 3150 INR/Bottle
Pfu Dna Polymerase Vial Product Specifications
- No
- High (35 exonuclease activity ensures low error rate)
- Clear or colorless solution
- New
- Up to 10 kb DNA fragments amplification
- PCR Amplification
- High accuracy, proof-reading activity, thermostable enzyme
- Pfu DNA Polymerase Vial
- No
- Vial size - typically 0.2 ml or 0.5 ml (as standard)
- Molecular biology reagent
- Store at -20C
- Approx. 1-5 g per vial
- Yes
- DNA polymerization during PCR
- Enzyme in storage buffer
- Laboratory reagent
- Manual (used in Laboratory protocols)
- High-fidelity DNA amplification
- 12-24 months (when stored properly)
Pfu Dna Polymerase Vial Trade Information
- Cash in Advance (CID), Cheque, Cash Advance (CA)
- 2-7 Days
- All India
Product Description
Being an eminent firm, we are offering a wide array of PFU DNA Polymerase Vial. It is to amplify DNA in the polymerase chain reaction. We provide a premium quality of solution of copying a new strand of DNA during each extension step. This vial is recommended for use in PCR and primer extension reactions. It enhances PCR product yields and increases target length capability. PFU DNA Polymerase Vial is avail at cost effective prices.
Specification
|
Packaging Type |
Vial |
|
Country of Origin |
Made in India |
|
Brand |
Aura Biotechnologies |
|
Form |
Liquid |
|
Packaging Size |
250 U/Vial |
|
Method |
PCR Enzyme |
|
Usage/Application |
Laboratory |
Exceptional Accuracy and Proofreading
Pfu DNA Polymerase is renowned for its high accuracy in DNA amplification, due to its 3-5 exonuclease proofreading ability. This feature significantly reduces errors, making it a preferred choice for researchers conducting cloning, site-directed mutagenesis, or other applications requiring precise amplification.
Thermostability and Reliability
Derived from Pyrococcus furiosus, Pfu DNA Polymerase operates efficiently at elevated temperatures, with an optimal reaction temperature of 72C and inactivation at 95C. Its thermostability ensures consistent results and enables amplification of longer DNA fragments without degradation.
Ready-to-Use and Contamination-Free
The supplied Pfu DNA Polymerase vial comes with a 10X reaction buffer and is assured contamination-free, devoid of DNase and RNase activity. Its clear, colorless solution in a portable vial is immediately ready for use in laboratory PCR protocols and offers reliable performance over an extended shelf life.
FAQ's of Pfu Dna Polymerase Vial:
Q: How do I use Pfu DNA Polymerase Vial for PCR amplification?
A: To perform PCR with Pfu DNA Polymerase, combine the enzyme (typically 2.5 U/l), supplied 10X buffer, DNA template, primers, and nucleotides. Set the reaction temperature to 72C for optimal enzyme activity, and finish with a final inactivation step at 95C for 10 minutes.Q: What are the benefits of using Pfu DNA Polymerase in high-fidelity PCR applications?
A: Pfu DNA Polymerase offers high accuracy due to its 3-5 exonuclease proofreading activity, significantly reducing mutation rates during amplification. This makes it ideal for applications such as cloning, site-directed mutagenesis, and any procedure where precise DNA replication is crucial.Q: When should I choose Pfu DNA Polymerase over other DNA polymerases?
A: Select Pfu DNA Polymerase when high-fidelity amplification is essential, especially for cloning, mutation studies, or generating DNA fragments up to 10 kb with low error rates. Its proofreading capability ensures higher accuracy compared to standard Taq polymerase.Q: Where should I store the Pfu DNA Polymerase Vial for optimal shelf life?
A: Store the Pfu DNA Polymerase Vial at -20C to maintain enzyme activity and ensure a shelf life of 12-24 months. Avoid repeated freeze-thaw cycles to preserve stability and performance.Q: What process ensures the enzyme is free from contamination?
A: Strict manufacturing protocols are followed to guarantee the enzyme is free of DNase and RNase contaminants. This ensures the supplied polymerase maintains integrity in sensitive molecular biology applications.Q: How do I inactivate the Pfu DNA Polymerase after PCR?
A: After PCR amplification, inactivate the Pfu DNA Polymerase by incubating the reaction tube at 95C for 10 minutes. This step ensures enzymatic activity ceases before downstream processing.Q: What are the typical applications for Pfu DNA Polymerase Vial in research laboratories?
A: Common applications include high-fidelity PCR, cloning, and site-directed mutagenesis, where precise DNA amplification is necessary. It is widely used in molecular biology labs, and distributed by exporters, suppliers, and manufacturers across India.Tell us about your requirement
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AURA BIOTECHNOLOGIES PRIVATE LIMITED
GST : 33AAMCA8110A1ZA
GST : 33AAMCA8110A1ZA
Contact Details
- Survey No. 270, Plot No. 1 and 2, Galaxy Road, Ayanambakkam, Chennai - 600095, Tamil Nadu, India
- Phone : 08045800169
- Mr PAZHANIMUTHU ANNAMALAI (Manager)
- Mobile : 08045800169
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