SYBR Green PCR Master Mix 2X

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Product Details:

Features Ready-to-use, high sensitivity, high specificity, fast results
Usage Type Laboratory, Molecular Biology
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Accuracy High
Function DNA quantification and detection
Instruments Type PCR and Real-Time PCR Instruments
Shelf Life 12 Months
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SYBR Green PCR Master Mix 2X Product Specifications

Color Light Green
Equipment Type PCR Master Mix
Operating Type Automatic
Shelf Life 12 Months
Real-Time Operation Yes
Material Liquid Reagent
Features Ready-to-use, high sensitivity, high specificity, fast results
Use Amplification and detection of DNA
Wall Mounted No
Technology SYBR Green-based Real-Time PCR
Usage Type Laboratory, Molecular Biology
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Condition New
Accuracy High
Portable Yes
Instruments Type PCR and Real-Time PCR Instruments
Function DNA quantification and detection

SYBR Green PCR Master Mix 2X Trade Information

Payment Terms Cash in Advance (CID), Cheque, Cash Advance (CA)
Delivery Time 2-7 Days
Main Domestic Market All India

Product Description

We are determined to execute high qualityof SYBR Green PCR Master Mix 2X. It is useful for genotyping applications. This range providesfaster setup with less pipetting and reduce the effect of contamination by its simple process. It is to set up controls and test different concentrations. SYBR Green PCR Master Mix 2X ensure less tube-to-tube variability. It is a method for performing real-time PCR analysis. It immediately used without any freezing issue.

Effortless Quantitative PCR Performance

Experience high sensitivity and specificity with this SYBR Green-based master mix. The convenient 2X concentrated format streamlines preparation, while the Hot Start Taq DNA Polymerase prevents non-specific amplification, ensuring reliable gene quantification and analysis for labs. Its compatibility with major PCR platforms enhances workflow efficiency across various research settings.

Optimized Buffer and Reliable Detection

Formulated with precise concentrations of MgCl2, dNTPs, and SYBR Green, this master mix delivers robust DNA amplification and fluorescent detection. The inclusion of enzyme stabilization components supports consistent performance during storage and multiple cycles, granting superior accuracy in quantitative measurements.

Flexible Packaging for Diverse Needs

Choose from a range of pack sizes tailored for different throughput requirements, whether for routine analysis or larger projects. The products portability and straightforward handling make it ideal for both established research laboratories and emerging molecular biology laboratories in India and beyond.

FAQs of SYBR Green PCR Master Mix 2X:

Q: How do I set up a reaction using SYBR Green PCR Master Mix 2X?

A: Simply combine the master mix and your DNA template in the recommended proportions. No additional reagents are required for standard qPCR protocols, facilitating rapid and easy setup.

Q: What types of quantitative PCR instruments are compatible with this master mix?

A: This master mix is compatible with all major real-time PCR platforms and instruments, making it versatile for use in various laboratory environments.

Q: When should SYBR Green PCR Master Mix 2X be stored, and under what conditions?

A: Store the master mix at -20C and avoid repeated freeze-thaw cycles to preserve potency. Under these conditions, the product maintains a shelf life of up to 12 months.

Q: Where can SYBR Green PCR Master Mix 2X be used?

A: Designed for laboratory settings, it is suitable for gene expression analysis, DNA quantification, and various molecular biology applications requiring real-time PCR technology.

Q: What benefits does the Hot Start Taq DNA Polymerase confer to the master mix?

A: The Hot Start enzyme enhances specificity and reduces non-specific amplification by remaining inactive until heated, leading to more accurate qPCR results.

Q: How does the use of SYBR Green dye allow for DNA detection and quantification?

A: SYBR Green intercalates with double-stranded DNA, emitting fluorescence that is directly proportional to the amount of amplified product, enabling precise quantification during PCR cycles.

Q: What sizes of SYBR Green PCR Master Mix 2X are available for purchase?

A: This product is offered in multiple pack sizes, including 1 mL, 5 mL, and 25 mL, allowing selection based on experimental scale and laboratory demands.

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