One Step Rt Qpcr Probe Kit

Name: One Step Rt Qpcr Probe Kit
Brand: AURA BIOTECHNOLOGIES PRIVATE LIMITED
Price: 10000 INR
Availability: InStock

10000 INR/Piece

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Product Details:

Instruments Type qPCR instrument compatible
Display Type Not applicable (display via qPCR instrument)
Function One-step reverse transcription and real-time amplification
Usage Type Multipurpose for clinical diagnostics, molecular biology, and research laboratories
Features One-step reverse transcription and amplification, high sensitivity, fast reaction, contamination minimization, robust performance
Shelf Life Up to 12 months (when stored under recommended conditions)
Accuracy High specificity and sensitivity in RNA detection
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One Step Rt Qpcr Probe Kit Price And Quantity

Price 10000 INR/Piece

One Step Rt Qpcr Probe Kit Product Specifications

Dimension (L*W*H) Kit dimensions may vary (typical: small reagent vials and packaging)
Equipment Type One Step Rt Qpcr Probe Kit
Instruments Type qPCR instrument compatible
Voltage Compatible with standard laboratory equipment
Wall Mounted No
Material Specialized Enzyme Solution, Buffer Components, dNTPs, RNase Inhibitor
Function One-step reverse transcription and real-time amplification
Display Type Not applicable (display via qPCR instrument)
Usage Type Multipurpose for clinical diagnostics, molecular biology, and research laboratories
Operating Type Automated thermal cycler required
Condition New
Real-Time Operation Yes
Noise Level Silent Operation
Accuracy High specificity and sensitivity in RNA detection
Shelf Life Up to 12 months (when stored under recommended conditions)
Power Consumption Depends on thermal cycler used
Power Source Laboratory equipment-powered
Portable Yes (compact and transportable packaging)
Color Kit components in colorless or light-colored vials
Features One-step reverse transcription and amplification, high sensitivity, fast reaction, contamination minimization, robust performance
Technology Real-Time Quantitative Polymerase Chain Reaction (qPCR) using hydrolysis probe
Use Detection and quantification of RNA targets in molecular diagnostics and research
Storage Instructions Store at -20C, avoid repeated freeze/thaw cycles
Measurement Range Wide dynamic range (10^1 to 10^7 copies)
Weight Varies by kit size, typically < 500g

One Step Rt Qpcr Probe Kit Trade Information

Payment Terms Cash in Advance (CID), Cheque, Cash Advance (CA)
Delivery Time 2-7 Days
Main Domestic Market All India

Product Description

Due to our proficiency of this domain, we have been carving a great niche in providing an exclusive array of One Step Rt Qpcr Probe Kit. It enables fast and highly reproducible procedures. Apart from this, provided kit is developing for probe-detection technology. It is applicable for modifying in food as well as cancer phenotyping. One Step Rt Qpcr Probe Kit is based on the detection of complementary strand extension in real time.

Optimal Sensitivity and Reliability

This kit detects RNA targets down to a single copy, providing outstanding sensitivity and specificity for diagnostic and research settings. Its high resistance to PCR inhibitors ensures reliable results even with challenging samples, making it suitable for clinical diagnostics, viral detection, and gene expression analysis.

Convenient One-Step Protocol

Streamline your workflow with one-tube, one-step reverse transcription and real-time qPCR. Simply combine the master mix, RT mix, and RNase-free water with your RNA sample; then run the assay on any compatible automated thermal cycler. The process greatly reduces contamination risks and overall hands-on time.

Robust Performance and Easy Storage

All components are vacuum-sealed in compact, insulated packaging to maintain enzyme stability during shipping. The kit is portable, easy to use, and has a shelf life of up to 12 months when stored at -20C. Its minimal handling requirements make it ideal for high-throughput screening and demanding lab environments.

FAQ's of One Step Rt Qpcr Probe Kit:

Q: How do I use the One Step RT qPCR Probe Kit for RNA detection?

A: Prepare your reaction by mixing the One Step qPCR Probe Master Mix, RT Mix, and RNase-free water with your RNA sample in a compatible vial. Load the mixture into an automated thermal cycler and follow the recommended cycling protocol in the user manual. The kit enables simultaneous reverse transcription and real-time PCR analysis in one step.

Q: What applications is this kit most suitable for?

A: This kit is ideal for viral RNA detection, infectious disease diagnostics, gene expression studies, and high-throughput screening in molecular biology research and clinical laboratories.

Q: When can I expect results from a typical run?

A: A complete run generally takes about 1 to 1.5 hours, enabling fast and efficient RNA detection and quantification.

Q: Where should the kit be stored to maintain stability?

A: Store all components at -20C, following the storage instructions provided. Avoid repeated freeze/thaw cycles to preserve enzyme activity and reliability.

Q: Which equipment is required to operate this kit?

A: You need an automated thermal cycler that is capable of real-time quantitative PCR (qPCR) analysis and compatible with hydrolysis probe technology.

Q: What benefits does the kit offer compared to multi-step protocols?

A: The kit's one-step protocol minimizes contamination risks, reduces handling time, and simplifies workflow. Its robust enzyme formulation ensures high sensitivity, rapid results, and exceptional accuracy in a variety of sample types.

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